---

This design used the genome downloaded from the Tutorial, using all defaults and the first 3 loci listed

CRISPR Application: CRISPRi
Target Organism NCBI Taxonomy ID: 511145
DownLoad GFF
Download FASTA

---

Target Region:

• Limit to coding regions
• Relative to loci: gene-D8B36_RS00005 gene-D8B36_RS00010 gene-D8B36_RS00015
• Starting point reference: First CDS Starting Base
• Starting point padding: 0
• Ending point reference: First CDS Starting Base
• Ending point padding: 100

Guide RNA Design Parameters

• PAM: NGG
• Target length : 20
• Miss-matches: 4
  
• Number wanted: 3
• Scaffold sequence: GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGGTGC
• Target strand: both

---

All genes had protospacers

All genes had enough protospacers

---

Download:
Results (.csv)
Target Regions (.fa)
Report (.txt)
All output files (.tar.gz)

---

View gRNA Design Results:
This may take a minute to load for whole genome designs